Observations placeholder
The vagus nerve and nicotinic receptors involve inhibition of HMGB1 release and early pro-inflammatory cytokines function in collagen-induced arthritis
Identifier
018143
Type of Spiritual Experience
Background
A description of the experience
J Clin Immunol. 2010 Mar;30(2):213-20. doi: 10.1007/s10875-009-9346-0. Epub 2009 Nov 5.
The vagus nerve and nicotinic receptors involve inhibition of HMGB1 release and early pro-inflammatory cytokines function in collagen-induced arthritis.
Li T1, Zuo X, Zhou Y, Wang Y, Zhuang H, Zhang L, Zhang H, Xiao X.
Author information
- 1Department of Rheumatology, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Abstract
OBJECTIVES:
The cholinergic anti-inflammatory pathway, a vagus nerve-dependent mechanism, inhibits cytokine releases in models of acute inflammatory disease. We investigated the efficacy and elucidated the possible mechanism of the cholinergic anti-inflammatory pathway on collagen-induced arthritis (CIA) in mice.
METHODS:
Fifty-six male DBA/1 mice were divided into four groups: control mice (sham vagotomy + phosphate-buffered saline; shamVGX+PBS), model mice (shamVGX+PBS+CIA), vagotomy mice (VGX+PBS+CIA), and nicotine (Nic) mice (shamVGX+Nic+CIA). We subjected mice to left-side cervical vagotomy 4 days before induction of arthritis. Mice in the nicotine group were injected with nicotine (250 microg/kg per day) 4 days before arthritis induction. Arthritis score was measured and histopathologic assessment of joint sections carried out. The concentration of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-10 in serum were evaluated by ELISA. Expression of high-mobility group box chromosomal protein 1(HMGB1) was evaluated by immunohistochemical staining of joints.
RESULTS:
Vagotomy exaggerated, whereas nicotine attenuated, clinical arthritis. Histopathologic findings confirmed that nicotine reduced infiltration of inflammatory cell and bone destruction. Expression of TNF-alpha and IL-6 decreased in nicotine-pretreated mice compared with model and vagotomy mice; IL-10 levels were not significantly different between the model group and nicotine group. Nicotine reduced the expression and translocation of HMGB1 in the inflamed joints of CIA mice.
CONCLUSIONS:
The cholinergic anti-inflammatory pathway has an anti-inflammatory role in the pathophysiology of rheumatoid arthritis (RA) via inhibiting HMGB1 release and early pro-inflammatory cytokines function. Study of this pathway could be used for RA therapy.
PMID:
19890701
The source of the experience
PubMedConcepts, symbols and science items
Concepts
Symbols
Science Items
CollagenActivities and commonsteps
Activities
Overloads
Autoimmune diseasesEhlers-Danlos syndrome
Rheumatoid arthritis
Suppressions
Acetylcholine and its ligandsNicotine
Tobacco