Observations placeholder
N-butanol extract of Capparis spinosa L. induces apoptosis primarily through a mitochondrial pathway involving mPTP open, cytochrome C release and caspase activation
Identifier
022054
Type of Spiritual Experience
Background
A description of the experience
Asian Pac J Cancer Prev. 2014;15(21):9153-7. N-butanol extract of Capparis spinosa L. induces apoptosis primarily through a mitochondrial pathway involving mPTP open, cytochrome C release and caspase activation. Ji YB1, Yu L. 1Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, China E-mail : yulei912@163.com.
BACKGROUND:
Capparis spinosa L., a Uygur medicine, had been shown to have anti-tumor activity in our early experiments with an N-butanol extract (CSBE) as its active fraction. However, the mechanisms responsible for its effects are not clearly understood. Here, we report that treatment of SGC-7901 cells with CSBE resulted in dose-dependent reduction of cell viability and induction of apoptosis.
MATERIALS AND METHODS:
To observe the inhibitory and killing effects of CSBE on SGC-7901, the SRB method was adopted, apoptosis being observed by electron microscopy. To clarify the mechanisms of apoptosis, Western blot and enzyme-labeled methods were used to examine the release of cytochrome c (Cyt c) and the activation of the caspase cascade.
RESULTS:
By electron microscopy, apoptotic morphologic changes were detectable after CSBE administration. In this study, it was also demonstrated that CSBE induced apoptosis in SGC-7901 cells by inhibiting mPTP open, mitochondrial cytochrome c release, caspase-9 and caspase-3 activation.
CONCLUSIONS:
The findings indicated that CSBE induces apoptosis through mitochondrial pathway.
PMID: 25422194